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Course Discription |
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This course will provide students with an introduction to modern molecular biology techniques, specifically recombination techniques including DNA and plasmid isolation from eukaryotic and prokaryotic cells, DNA purification, restriction digest and gel electrophoresis, bacterial transformation, reverse transcription, polymerase chain reaction (PCR and RT-PCR) and cloning DNA fragments. The lectures and assigned readings will cover the theory and experimental applications of these molecular techniques. |